The nano-zinc titanate (ZnTiO₃) photocatalyst was prepared by hydrothermal-assisted sol-gel method. Rhodamine B was taken as the target degradant. The effect of initial concentration of Rhodamine B (RhB) on the degradation was analyzed by kinetic model. ZnTiO₃ was characterized by SEM, XRD, XPS and UV-Vis DRS. The degradation mechanism was analyzed by free radical trapping experiments. The results show that ZnTiO₃ is a pure hexagonal phase with a spherical shape and a particle size of about 50 nm. Under the condition of catalyst dosage of 1 g·L^-1, initial concentration of RhB of 5 mg/L and pH of 3, the degradation rate of RhB was 93.2% after photocatalytic reaction for 150 min. Its kinetic equation is k=0.132C₀^-1.253. In the degradation process of ZnTiO₃ photocatalyst, ·OH, h⁺, ·O₂^- all play a catalytic role, and the amount of ·OH and h⁺ is similar and more than ·O₂^-, indicating that ·OH and h⁺ play a major role in the catalytic reaction.