To reveal the thermal comfort characteristics and thermal environment requirements of outdoor pedestrians and cyclists in hot and humid areas in summer, the methods of environmental parameters testing and questionnaire survey was used to investigated the thermal environment status, thermal comfort, thermal environment preference and thermal environment acceptability of pedestrians and riders, then correlation analysis was carried out. The results show that with the change of shading rate, thermal sensation of pedestrians are more sensitive than that of cyclists, and the comfort level is lower than that of cyclists. The main uncomfortable factors are excessive solar radiation and temperature. The lower the SET* value, the more comfortable pedestrians and cyclists feel. The thermal requirement for pedestrians is that SET* ≤ 30.2℃,TSV ≤ 1.4, for cyclists SET* ≤ 32.9℃,TSV ≤ 1.5, Pedestrians have higher requirements for thermal environment.