The dynamic resilient modulus of subgrade soil is an important parameter in design of asphalt pavement structure. Accurate acquisition of the dynamic resilient modulus of subgrade soil is always one of the important research subjects in the field of road engineering.In this paper, based on the investigation of the relevant results of domestic and foreign subgrade soil dynamic resilient modulus testing methods, a dynamic resilient modulus testing method for subgrade soil considering influence of the loading and unloading duration has been established through finite element numerical and exploratory tests. Two kinds of typical subgrade soils were selected to carry out dynamic resilient modulus tests considering the loading and unloading duration of the vehicle load. The experimental results show that the dynamic resilient modulus of the two kinds of soil increases with the increase of confining pressure, and decreases with the increase of cyclic deviatoric stress and loading time.With the increase of loading time, the dynamic resilient modulus of subgrade soil can be reduced by 34.6%, and the influence of loading time cannot be ignored.Under different loading time, the influence law of the dynamic resilient modulus of subgrade soil with confining pressure and cyclic deviatoric stress is basically same.