In order to study the moment-resisting performance and seismic behavior of the interior joints with wooden peg of Chuan-Dou type wooden frame, two full-scale interior beam-column joint specimens of transverse frame and two that of longitudinal frame were designed and fabricated. Monotonic loading and low cyclic loading tests were carried out on the two types of joints, respectively. The failure mode, moment-resisting capacity, flexural stiffness, ductility and energy dissipation of the two types of joints were obtained. The results showed that the failure mode of transverse interior joint was the embedment plastic deformation on the mortise and tenon contacting area as well as the bending-shear combined deformation of wooden peg, while longitudinal interior joint was broken off at the neck of tenon. The initial stiffness of the transverse interior joint was higher than that of the longitudinal interior joint. The moment-resisting capacity of transverse interior joint could reach two times of that of longitudinal interior joint. The hysteretic loops of the two types of joint exhibited obvious squeezing and slip. The energy dissipation of the transverse interior joint was higher than that of the longitudinal interior joint. Both the two types of joint exhibited good deformation capacity, the transverse interior joint exhibited good ductility, while the longitudinal interior joint exhibited poor ductility.The longitudinal interior joint in the frame is the weakest part and need to be noted on the safety maintenance of the Chuan-Dou type wooden frame structure.