Extraction of potato urease and improvement of aeolian sand based on EICP Technology

doi:10.11835/j.issn.2096-6717.2022.048

Home > Archive>Volume 45, Issue 2, 2023 >74-80. DOI:10.11835/j.issn.2096-6717.2022.048

Extraction of potato urease and improvement of aeolian sand based on EICP Technology
DOI:
                        10.11835/j.issn.2096-6717.2022.048
                    
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                        [cstr]
                    
Author:
                        CHU Wenjie1CHU Wenjie
School of Civil Engineering; key Lab. of Geological Hazards and Geotechnical Engineering Defense in Sandy and Drought Regions, Inner Mongolia University of Technology, Hohhot 010051, P. R. China
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LI Chi1LI Chi
School of Civil Engineering; key Lab. of Geological Hazards and Geotechnical Engineering Defense in Sandy and Drought Regions, Inner Mongolia University of Technology, Hohhot 010051, P. R. China
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WU Huimin2WU Huimin
Inner Mongolia Water Conservancy and Hydropower Survey and Design Institute, Hohhot 010021, P. R. China
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GAO Yu1GAO Yu
School of Civil Engineering; key Lab. of Geological Hazards and Geotechnical Engineering Defense in Sandy and Drought Regions, Inner Mongolia University of Technology, Hohhot 010051, P. R. China
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Affiliation:1.School of Civil Engineering; key Lab. of Geological Hazards and Geotechnical Engineering Defense in Sandy and Drought Regions, Inner Mongolia University of Technology, Hohhot 010051, P. R. China;2.Inner Mongolia Water Conservancy and Hydropower Survey and Design Institute, Hohhot 010021, P. R. China
Clc Number:TU441
Fund Project:Inner Mongolia Natural Science Foundation Project (No. 2019MS05075); Key Technology Research Project of Science and Technology Plan of Inner Mongolia Autonomous Region (No. 2021GG0344).

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Abstract:

Based on enzyme induced calcium carbonate precipitation (EICP) technology, plant urease was used to improve and strengthen desert aeolian sand. In order to find a cheap and simple urease extraction method, potato was used as the raw material, deionized water, glycerol aqueous solution and ethanol aqueous solution were used as extraction solution, and the plant urease in potato was separated and extracted by crushing, refrigeration and high-speed centrifugation, so as to study the influence of different extraction solutions on the extraction effect of urease; The results showed that the best of the three extracts was ethanol solution. When the solid-liquid ratio was 1:6 and the concentration of ethanol solution was 30%, the urease activity value was the highest and the extraction effect was the best. In the improvement experiment of aeolian sand, a hard crust is formed on the surface of the specimen and is of high compressive strength, and the unconfined compressive strength increases gradually with increase of cycles of soaking urease.

Key words:enzyme induced calcium carbonate precipitation (EICP);plant urease;urease extraction;urease activity;windbreak and sand fixation

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褚文杰,李驰,武慧敏,高瑜.土豆脲酶提取及基于酶诱导碳酸钙沉淀技术对风积沙改良的方法[J].土木与环境工程学报（中英文）,2023,45(2):74~80

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Received:August 10,2021
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Online: March 20,2023
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