In geotechnical engineering, the small-strain shear modulus of sand G_max is an important mechanical parameter. The isotropic consolidation tests were carried out on artificial glass sand, combined with multi-direction bender element tests. The effects of MICP reinforcement methods and bender element arrangement on G_max of glass sand were investigated. The test results show that the development pattern of shear wave velocity V_S, small strain stiffness G_max and stiffness anisotropy G_HH/G_HV of glass sands reinforced by different methods are different. After MICP reinforcement, the G_max of glass sand is increased. Among them, “low pH one-phase method + direct back pressure after injection of reaction solution” has the greatest G_max increase, and the small-strain stiffness ratio (Gmaxload /Gmaxunload) has the maximum decrease. Due to the nonuniformity reinforcement, the placement height of bender element on the side wall also has a certain influence. Based on the test results in this study, it is recommended to arrange the HH and HV bender elements at the same plane to further discuss the effect of MICP on small strain stiffness anisotropy. Otherwise, the test error caused by reinforcement inhomogeneity can not be avoided.