The main objectives in this paper are to study mechanical performances of reinforced concrete two-way slab with different stud parameters.The experimental study on 7 reinforced concrete two-way slab-column connections with punching shear studs were completed.The main parameters were spacing of stud, the diameter of the stud, the height of the stud, the strength of the stud and the arrangement of the stud.The entire process of damage of slab-column connections with studs was simulated by using static loading method. By comparing and analyzing the test results of specimens with studs,the results show that increasing the diameter of the stud,the height of the stud or the strength of the stud can improve the anti-punching shear performance of the specimen.The increase in diameter is the most obvious, and the increase in deformation is 36%.Increasing stud spacing and reducing stud height are both not good for the improvement of the test, and the load-bearing capacity and the deformation are reduced.By comparing three different stud arrangements it is found that using radiation arrangement with the same number of studs and orthogonal arrangement with increasing the number of studs are both conducive to the improvement of the specimen anti-punching shear capacity,and the former was more economical.And then the reliability of the test results was verified by comparing the calculated results of relevant specifications with the test results.The results show that the safety reserve of specification calculation results of slab-column structure with studs is enough. However,it is not enough to reflect the nature of test results,especially contributions of the stud to anti-punching shear performance of slabs are underestimated.