In geotechnical engineering, the small-strain shear modulus of sand Gmax is an important mechanical parameter. The isotropic consolidation tests were carried out on artificial glass sand, combined with multi-direction bender element tests. The effects of MICP reinforcement methods and bender element arrangement on Gmax of glass sand were investigated. The test results show that the development rules of shear wave velocity VS, small strain stiffness Gmax and stiffness anisotropy GHH/GHV of glass sands reinforced by different methods are different. After MICP reinforcement, the Gmax of glass sand is increased. Among them, "low pH one-phase method + direct back pressure after injection of reaction solution" has the largest Gmax increase, and the small-strain stiffness ratio (Gload max /Gunload max) has the largest decrease. Due to the nonuniformity reinforcement, the placement height of bender element on the side wall also has a certain influence. Based on the test results in this study, it is recommended to arrange the HH and HV bender elements at the same plane to further discuss the effect of MICP on small strain stiffness anisotropy. Otherwise, the test error caused by reinforcement inhomogeneity can not be avoided.