Currently, filtration is a commonly used treatment method in wastewater treatment plants. Based on the differences in the materials and properties of filtration media, filtration can be classified into four main categories: microfiltration, ultrafiltration, nanofiltration, and reverse osmosis. The objective of this study is to develop a novel type of porous CaCO₃ membrane-filter through the process of microbial-induced calcium carbonate precipitation (MICP). The membrane-based filter is designed to remove microorganisms as well as eliminate extracellular polymeric substances (EPS) from solution. The experimental findings demonstrate that MICP driven porous CaCO₃ filter is highly efficient in eliminating Escherichia coli, Brachybacterium sp., and activated sludge, with removal rates of about 99.998%, 99.983% and 99.996%, respectively. It also exhibits superior EPS removal capability compared to general filter paper. Additionally, the MICP-treated bioslurry possesses desirable pore space, non-blocking features, and high permeability.