In the practical application of microbial induced calcium carbonate precipitation (MICP), urease-producing bacteria (UPB) cannot tolerate high salt concentrations in cementation solution (CS). Increasing the salt tolerance of UPB to CSs can improve the efficiency and the application scope of MICP. In this study, a domestication method was adopted to increase the salt tolerance of UPB (Sporosarcina pasteurii). The growth characteristics of the bacteria in the domestication medium, the calcium ion precipitation rates of untamed and domesticated strains, and the strength characteristics of biocemented sand columns were evaluated. The results indicate that the bacteria's growth rate was slowed down in the domestication medium. The salt concentration of the CS, which can be tolerated by the domesticated bacteria (DB) increased by approximately 0.25 mol/L. The specimens treated with DB exhibited an approximately 2-fold increase in unconfined compressive strength (UCS) at high concentrations of CS rather than untamed bacteria (UB). This research demonstrates the potential of domestication to optimize the biotreatment process in an environment of high salt.