The proliferation and division of cells are the most basic reaction. The effects of this reaction are the fact that the cell cycle was been influenced. Normal cells will change into tumor cells when its cell cycle running out of control; on the other hand, it will become feeble and die when its cell cycle stopping. Methods of synchronization and detection with flow cytometer of rat hepatocellular carcinoma cell line (HTC) are established in our laboratory. The results show that: as HTC cell is in the logarithmic period, the synchronous 72.10% G 1 phase and 98.94% S phase cells are achieved through thymine 2 desoryriboside and cochicine sequential blockage method and double thymine 2 desoryriboside blockage method respectively. It is proved that those results ware stable and reliable.