Extracting features from biomedical signals to provide some decision support for medical diagnoses is an important aspect in the development of biomedical engineering.Analyzing power spectra of pulse signals for 15 heroin addicts and 15 healthy persons and calculating the power distributions on specified frequency bands for every power spectrum based on spectral estimation in this paper,we find that the significant difference of power distributions exists between the heroin addicts and the healthy persons.A primary criterion is also obtained,upon which the 13 cases of 15 heroin addicts are identified.The research result of this paper shows that the frequency domain analysis for pulse signals of heroin addicts is really an effective method.