To research role of biomedical engineering in treatment of Gram_negative bacterial sepsis,and chemical configuration of endotoxin and role of endotoxin in septic shock and multiple organ failures were analyzed.Effects of various biotechnology in treatment sepsis were compared. There are relationship between in endotoxin and sepsis,no effective medication for sepsis. Hemoperfusion with Polymyxin B immobilized absorbents could remove endotoxin of blood, decrease flammatory mediators(eg,tumor necrosis factor-alpha and interleukin-1) of blood ,and improve hemorheology. Polymyxin B immobilized absorbents are important in treatment patient of sepsis by its specific absorbing endotoxin .