The results of studies of Antineopalston A10 show it's degradation products, such as glutamines and isoglutamines, having antitumor activity. According to these, some N'-phenylacetyl-N-substituted glutamines are designed and synthesized for researching their antitumor activity. In the syntheses of these compounds, the acid azides are prepared first, which then react with amines at low temperature to transform to the target molecules. The mild reaction conditions and good yields and the optical activity maintaining are the merits of this method. Evaluation of antitumor activity of the target molecules is carried out by MTT method to L1210 cell strain. The result showes some compounds can inhibit growth of the cancer cells.