The relative characater of silk fibroin in biomedical application is discussed ,which focuses on the problems about biocompatibility and degradation mechanism while silk fiber is used in vitro .As the result,it is easier to keep the biocompatibility of silk fibroin by the characater of multiple styles of crystallizes and construct the net structure. The modification of membrane structure of other natural material is also discussed. as well as the chemical way in the main application statue in drug-loading material , artifical organ or tissue scaffold. The degrading of silk fibroin in vitro is also controlled by the temperature and mechanical enviroment as well as the chemical component,and it is should be more comprehensive with the factors of biomechanic and biorheology as well as that of biochemical taken into considered in the investigation of transformation mechanism of silk in vitro.