Study on Freezing Storage of Human Dermal Papilla Cells
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Abstract:
To explore a safety method of freezing storage of human dermal papilla cells not influencing its biological activity. The enormous dermal papillae isolated newly were freezed with cryoprotective agent including dimathyl sulfoxide and storaged in liquid nitrogen after sinking gradually. At the 1 month, 2 month and 6 month after submersing in liquid nitrogen, the freezing dermal papillaes got revivfication after thawing, inoculated and raised in Dulbecco's Modified eagle's medium. The rate of dermal papillaes attaching to the wall and the cell growth behaviour were observed. Similar to that of islated newly, the rates of dermal papillaes attaching to the wall at 2 days after culture were 90 percents to 95 percents after freezing for 1 month, 2 months and 6 months. The dermal papillae also can culture for dermal papilla cell which exhibites a polygonal cellular morphology and a tendency to form pre-confluent multi-layered cluster after restored in liquid nitrogen. It was approximately 15 days to approach cell fusion in culture bottle. Conclusion It is suggested that freezing storage of human dermal papillaes has no side-effect in their biological characteristics and is worth application in the series experiment of DPC .
