This article introduces the use of low temperature in medical science. The low temperature medicine includes cryosurgery, cryopreservation, low body temperature and cryodrying. Hypothermia-agent of freezing therapy is generally used by liquid nitrogen. It is economical, convenient and easy to operate. It has been extensively applicated to clinical medicine, such as treatments of benign tumor and malignant tumor effectively. Additionally, cryopreservation of blood, sperm, tissue or organ is also operated perfectly, but it needs to be further studied and developed.