In the system of infrared imaging acquisition, it is necessary to improve data transfer to satisfy the characteristics of high speed and real time. So,this paper introduces PCI bus and PCI inerface to imaging acquisition. The protocol of data transfer of PCI local bus and the structure of PCI9050 are studied in detail, and the IRFPA data acquisition system are described based on the protocol of data transfer of PCI local bus. The system adopts frame buffer to make slow transmission between peripheral circuit and PCI bus.CPLD is also used as a master of logic time control?