The Extraction,Isolation and the Composition of Polysaccharides From Ganoderma Apparatus and Poria Cocos
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Abstract:
This paper reports that the crude polysaccharides were extracted with hot water and precipitated with alcohol from the fruitbody of mushroom Ganoderma apparatus and Poria cocos. The free proteins were removed from crude polysaccharides by sevag method, and then the polysaccharides were isolated and purified by Sephadex G-15 gel column chromatography. Each three pure polysaccharides (Ga-1,Ga-2,Ga-3 and Pc-1,Pc-2,Pc-3) were obtained by precipitating with different concentration alcohol. By using the method of TLC, we proved that three fractions of Ganoderma apparatus polysaccharides are composed of glucose (Glu), arabinose (Ara), mannose (Man), fructose(Fru),et al. and the three fractions of Poria cocos polysaccharides are composed of glucose (Glu), mannose (Man) and ribose (Rib),et al.
