Biological Mass Spectrometry for Macromolecules:Approaches and Applications
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Abstract:
Biological Mass Spectrometry (BioMS) has been developed to solve the analytical problems of biological substances in life sciences, especially for structural analysis of biological macromolecules such as proteins, nucleic acids, amyloses. The development of BioMS depends primarily on soft ionization techniques including electrospray ionization mass spectrometry (ESI-MS), matrix assisted laser desorption ionization mass Spectrometry (MALDI-MS), Fast Atom Bombardment mass Spectrometry (FAB-MS), Ion Spray Ionization Mass Spectrometry (ISI-MS), Atmospheric Pressure Ionization mass spectrometry (API-MS). Among them ESI-MS and MALDI-MS was proposed to analyze biological macromolecules by Prof. Dr. John B. Fenn and Mr. Koichi Tanaka pioneered the successful application of their techniques to biological macromolecules and shared one half of the Nobel Prize in Chemistry for 2002. BioMS has become the activest field and promoted the analytical theory and testing technology. Some approaches and applications of BioMS were briefly introduced and properly discussed in the structural analysis of proteins , nucleic acids , amyloses and biomacromolecules and some other complexmolecules, which are useful for geneomics, proteomics, glycomics and other bioomics and chemomics.
