Xenograft bone such as Kiel bone has long been used as a grafting materials,but the results of Kiel bone application in clinic are still controversial due to it's some drawbacks. To investigate the novel procession method of xenograft bone, the physicochemical characterization of Kiel bone and it's relationship with the clinical application are studied. Kiel bone is prepared from bovine femoral cortical bone according to Salama's methods.The bone specimens are characterized with a number of physicochemical methods such as FTIR spectroscopy, X-ray diffraction spectroscopy , differential scanning calorimetry (DSC) and uniaxial tension mechanical tests. The result indicates that Kiel bone is composed of hydroxyapatite and organic component ,and the deproteination of Kiel bone is imcomplete, but processes with hydrogen peroxide, the collagen is denatured which reduce the mechanical properties of materials. The immunogenicity of Kiel bone is weakened since the main antigenic protein is removed and collagen is denatured, which not only weakens the strength of materials but influences on it's physiological property, all make the osteoinductive properties of materials unideal. To overcome these problems, it is necessary to develop the novel procession method of the xenogeneic bone.