The destruction of rock mass arises from opening rock possibly, the main outside-factor of influence rock slope stability is groundwater and water seepage. To analyze the relationship of unloading and permeability coefficient, the method and step of experiment of unloading rock mass are put forward. The curve relationship of the seepage-unloading of different phase is found by experiment. According to the experimental result, the equation of the seepage-stress of unloading is established, the variational rule of the unloading and permeability coefficient is discussed. The permeability coefficient is almost unchanged in the elasticity phase of unloading process. The permeability coefficient changes very much in the plastic phase of unloading process, as the unloading is 80% of ultimate unloading especially, and the permeability coefficient is increasing very much.