Primary Study on Sound Effecting the Gene Expressions of Arabidopsis thaliana
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Abstract:
The differential expressions of Arabidopsis thaliana seedlings under sound stimulation are studied primarily by applying mRNA reverse transcription differential display PCR(DDRT-PCR) with silver staining.To identify the truth of the differentially expressed cDNA fragments,Northern dot is performed.Among six differential expression gene fragments(SA3,SG2-1,SG2-2,SG7-1,SG7-2 and CA2) acquired,SA3,CA2 and SG7-1 are found to be positive fragments.SA3 is differentially expressed and SG7-1 is advantaged expressed,while CA2 is restrained by the sound wave.The(results) imply that plant has response to sound stimulation at gene level.