By the experimental study on a full-dimensioned prestressed frame of two spans, the relative factors are analyzed such as relative height of equivalent compression zone in critical section, secondary moment, ratio of beam rigidity to column and development of cracks in the top of columns etc., which influence the moment distribution of the post-tensioned prestressed concrete frame from the side of internal force redistribution. It is believed that that the factors of relative height of concrete equivalent compression zone and secondary moment influence the moment modulation in most degree, and ratio of column rigidity to beam also plays a part in the moment modulation to some extent. Second, the plastic joints of frame with excessive spans exit in the span which endure the whole load, at last the whole frame is destroyed because of the destruction of the loading frame, the influence to the non-loading span is little.