Overall effects of HRT ( 11 h, 9 h, 8 h, 6 h ) on the Spiral up-flow reactor (SUFR) system are analyzed. The results of the operation under different HRT condition shows that the removal efficiencies of COD, TN and TP decreased not obviously with decreasing HRT when HRT is longer than 8h. Anaerobic retention time, anoxic retention time and aerobic retention time were 1.51 h,2.25 h and 4.24 h,respectively. The removal efficiencies of COD, TN and TP is kept over 93%,87% and 90.2%, respectively. Although the removal efficiencies changed obviously when the HRT was 6 h. Anaerobic retention time, anoxic retention time and aerobic retention time are 1.13 h,1.69 h and 3.18 h,respectively. COD-removal efficiency is always up to 85%, the removal efficiencies of TN and TP is kept over 76% and 72%,also has upper removal efficiencies. It is indicated that the SUFR system, which is similar to plug-flow-reactor, has strong stability and can compensate negative effects of increasing hydraulic loading.