The paper explains the data transfer mechanism based on the PASS-THRU channel of S5933, and gives a method to develop data acquisition system based on PCI bus, the problem of designing the PCI interface controller is avoided, saving time and cost of development production. The use of CPLD makes the stucture of the system more integrated. The 32-bit data acquisition system,the speed of data transmission is faster,the CPU resource and PCI bus resource are spared.