The bioreactors are widely used in the cell culturing in tissue engineering. For the construction of similar structure and function tissue as in vivo, people begin to adding mechanical stimuli to the bioreactor. That is not only crucial for the fabrication of engineered tissues in vitro, but also can facilitate our understanding the characters of the live tissue. The review summarizes the major approaches for cell culture, using mechanical stimuli and bioreactors. Furthermore, the suggestions on the future developing of bioreactors are provided.