It has been reported that two Schiff base transitional metal complexes with morpholine ring were synthesized and characterized,and two complexes with same agent and different metal ion were used in the catalytic hydrolysis of p-nitrophenyl picolinate acetate as a simulant hydrolase of carboxylic acid ester.Kinetics and mechanism of PNPP catalytic hydrolysis are studied.The kinetic model of PNPP catalytic hydrolysis is proposed.The kinetic and the thermodynamic constant on the catalytic reaction are calculated.The result of the study shows: two complexes revealed good catalytic activity in PNPP catalytic hydrolysis.The rate of the PNPP catalytic hydrolysis is increased following increasing pH value of the buffer solution and affected by the polarization action of metal ion of complex.