In order to study the principle of liquid sterilization by high-pressure gas,the Orthogonal experiment based on the value index of time,pressure,solution concentration and sterilizing medium was conducted.The experimental results confirm the rupture mechanism by comparing the rate of inactivation among the different medium.The dissolved CO_2's acidity has no influence on the rate of sterilization.It also show that the rate of sterilization will increase when time,solution concentration and pressure raise.