A rapid method for DNA extraction from Pennisetum hybrid was developed.Using the method,leaves of the Pennisetum hybrid was treated by the 0.05 mol NaOH solution and heated in the boiling water for 10 min,followed by the neutralization with TE solution(ph=3.0),and the DNA extraction was used for RAPD-PCR and SSR-PCR analysis.The result indicates.The quality of the DNA by the simplified method is amount to DNA extracted by the CTAB method,and the DNA extracted by this method an meet the requirement of molecular marker analysis based on PCR technology.Combined with the 96-well plate,it can realize the high-throughput DNA extraction.