To make up the vacancy of the evaluation methods for ATV ride in the world, the pratical feature of ride comfort of ATV are studied and analyzed. Based on the ISO2631 and ISO5349, the objective methods of ride com-fort of ATV are proposed. This method uses the total root mean square of weighed of three axles to evaluate the vibration of the handle and seat. Road tests and experiments on rolling drum experiment table with the test system on ride comfort of some typical ATVs are conducted, the result of the two tests are matched each other, so the evaluation method can well reflect the effects of the vibration on human body.