Cobalt phthalocyanine （CoPc）was prepared from phthalodinitrile and cobalt sulfate via microwave irradiation. The Resonance Rayleigh scattering（RRS）was enhnaced by the interaction of sulfonated CoPc and bovine albumin（BA）. An RRS peak formed at 460 nm with intensity proportional to the concentration of BA. A simple and rapid method of RRS for the determination of BA thus was established. The linear range is 0.1 ug/mL to~6.0 ug/mL. The detection limit for BA is 5.73 ng/mL.