The essentiality of humaninformation system interaction efficiency was analyzed under the computerization environment of enterprises. Information processing ability was measured from three dimensions: working memory, cognitive skill, and computer selfefficacy. The experimental method was used to study the influence of information processing ability on humaninformation system interaction efficiency. Multiple regression results show that cognitive skill is the main factor influencing interaction efficiency for skillbased tasks, working memory is the main factor for rulebased tasks, and working memory has no significant influence on interaction efficiency for knowledgebased tasks. Working memory and computer selfefficacy are the main factors influencing interaction efficiency for total tasks.