Identification of Pinellia ternata by high performance liquid chromatographic fingerprinting
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Abstract:
The chromatographic fingerprint of Pinellia ternata was established by high performance liquid chromatography (HPLC) using an Agilent C18 (5 μm, 4.6×250 mm) column; CH3OH∶H2O as elution solvents; an ultraviolet detector at 254 nm; a flow rate of 1.0 mL/min. The guanosine peak was used as the reference and the relative retention value α and the relative area Sr as the parameters for the survey of the chromatographic fingerprint. Nine copossessing peaks were selected as the charaterizing fingerprint peaks to distinguish Pinellia ternata from false Pinellia ternata. The relative retention time of the 9 peaks were 1.00, 1.12, 1.50, 1.56, 1.60, 1.67, 1.71, 1.81 and 1.87, respectively. These peaks can be used to identify the quality of Pinellia ternata.
