Six haptens of pyrethrins are synthesized by preparing carboxyl of a ketone group of fivemembered rings．Six complete antigens are made by conjugating haptens to Bovine serum albumin (BSA) using a carbodiimide method．Ultraviolet spectrophotometry is used to demonstrate that the molecule conjugation ratios of Pyrethrin I-II, Cinerin I-II, and Jasmolin I-II to BSA are 6∶1, 13∶1, 9∶1, 11∶1, 7∶1, and 9∶1, respectively．The complete antigens are used as immunogens to immunize BALB/c mice to prepare antiserum．The antibody titers exceed 105 when measured using indirect enzymelinked immunoassay (ELISA). The polyclonal antibodies against pyrethrins are obtained via saturated ammonium sulfate precipitation．This research is may improve enzymelinked antibody detection and immunosorbent assay of pyrethrins．