LI Zhengguo
Chongqing Engineering and Technology Research Center for Fungal Insecticides; ,Genetic Engineering Research Center, BioEngineering College, Chongqing University, Chongqing 400030, P. R. ChinaFU Yongyao
Chongqing Engineering and Technology Research Center for Fungal Insecticides; ,Genetic Engineering Research Center, BioEngineering College, Chongqing University, Chongqing 400030, P. R. ChinaDUAN Wei
Chongqing Engineering and Technology Research Center for Fungal Insecticides; ,Genetic Engineering Research Center, BioEngineering College, Chongqing University, Chongqing 400030, P. R. ChinaYANG Yingwu
Chongqing Engineering and Technology Research Center for Fungal Insecticides; ,Genetic Engineering Research Center, BioEngineering College, Chongqing University, Chongqing 400030, P. R. ChinaDENG Wei
Chongqing Engineering and Technology Research Center for Fungal Insecticides; ,Genetic Engineering Research Center, BioEngineering College, Chongqing University, Chongqing 400030, P. R. ChinaXIA Yuxian
Chongqing Engineering and Technology Research Center for Fungal Insecticides; ,Genetic Engineering Research Center, BioEngineering College, Chongqing University, Chongqing 400030, P. R. ChinaSix haptens of pyrethrins are synthesized by preparing carboxyl of a ketone group of fivemembered rings．Six complete antigens are made by conjugating haptens to Bovine serum albumin (BSA) using a carbodiimide method．Ultraviolet spectrophotometry is used to demonstrate that the molecule conjugation ratios of Pyrethrin I-II, Cinerin I-II, and Jasmolin I-II to BSA are 6∶1, 13∶1, 9∶1, 11∶1, 7∶1, and 9∶1, respectively．The complete antigens are used as immunogens to immunize BALB/c mice to prepare antiserum．The antibody titers exceed 105 when measured using indirect enzymelinked immunoassay (ELISA). The polyclonal antibodies against pyrethrins are obtained via saturated ammonium sulfate precipitation．This research is may improve enzymelinked antibody detection and immunosorbent assay of pyrethrins．
李正国,符勇耀,段伟,杨迎伍,邓伟,夏玉先.除虫菊酯人工抗原的合成及鉴定[J].重庆大学学报,2009,32(2):233~238
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