AMT (Automatic Manual Transmission) comprehensive performance test is one of the main means to develop and evaluate AMT. An overall scheme of the data acquisition and communication system for AMT comprehensive performance testbench is proposed according to the requirement of the testbench, and a communication network of data acquisition and communication system is built. The hardware and software of data acquisition system are designed. The experiments show that the system meets the requirement of data acquisition and communication system for AMT comprehensive performance testbench and it lays a foundation for AMT comprehensive test research.