A chip with smaller size and higher pixel density was designed to increase the chip’s pixel density in subretinal prosthesis. This chip was designed on 0.35 μm standard CMOS(complementary metal oxide semiconductor) technology of CHRT company, while the double inverter relaxation oscillator was chosen as the basic pixel circuit. By creating layout on Cadence software, the circuit was debugged and simulated. The experiment results show that the pixel circuit provide effective pulse signals whose values and frequencies can vary with photocurrent to stimulate retinal nerve cells. For the simulated layout, the pulse width was 0.26 ms, the frequency was 18～503 Hz, the pixel size was 65 μm×65 μm, and the chip size was 1.1 mm×1.1 mm. All of the parameters can satisfy the requirement to stimulate retinal nerve cells in physiology. The experiment results provide a good foundation for the following research.