This study investigates the mercury distribution characteristics at different particle sizes, leaching characteristics with different impact factor such as the different time, the different ratio of liquid to solid and the different acidity, leaching toxicity and speciation of mercury in Municipal Solid Waste Incineration (MWSI) fly ash in Three Gorges area. The results show that the average content of mercury in the fly ash is 8.52 mg·kg1. The content of mercury in the fly ash is higher at the particle size of 100 μm, and which increases with the decreas of the ash particle size. Mercury leaching content closely relates to environmental conditions. The leaching content of mercury increases with the increase of pH and L/S, while it decreases with the leaching time. The content of mercury at oxidable form and the residue form are high, and whith at exchangeable form and reducible form is relatively low.