Based on the practice of machine tool remanufacturing, a software system is developed to support the processes of comprehensive testing and evaluation for machine tool remanufacturing. The software is composed of function models of comprehensive testing and analyzing, redesign and evaluationbased optimization. Based on the test of used machine tools, the redesign scheme of machine tool remanufacturing is presented. In addition, the scheme is evaluated in terms of technology, economy, resource consumption and environmental impact. An example of the used lathe remanufacturing is given to illustrate the application of the system.