The paper selects septic tank nightsoil sludge as the study object, and conducts three treatments for nightsoil sludge anaerobic digestion reduction under 35 ℃ including CK treatment(without HBHII compound microorganism), T1 treatment(adding 0.005% HBHII compound microorganism in the original nightsoil sludge system) and T2 treatment(adding 0.005% HBHII compound microorganism after the nightsoil sludge was sterilized by pasteurism). It investigates the removal rate of total solid (TS), volatile solid (VS) and COD of the three treatments, and analyzes the main contents of nightsoil sludge before and after anaerobic digestion and the digestion efficiencies of them. The results indicate that the removal rates of TS, VS and COD of T1 are 46.03%, 49.96% and 47.06%, respectively, and there are 14.89%, 16.26% and 15.53% higher than CK, 6.63%, 8.02% and 11.90% higher than T2, respectively. There are significantly positive (p<0.01) relations between T1 and CK, and T1 and T2 in the removal rate of TS, VS and COD. The nightsoil sludge digestion efficiencies of CK, T1 and T2 are 34.69%, 51.09% and 43.05%, respectively and that of T1 significantly positive(p<0.01) differs from CK and T1 treatment. The results indicate that the corporate effect of the adding HBHII compound microorganism and the original microorganism of the nightsoil sludge system are helpful to the reduction of septic tank nightsoil sludge and it is feasible for directly adding HBHII compound microorganism to septic tank for the reduction of the nightsoil sludge in it.