Analysis of the extracelluar polysaccharide’s fermentation processand the waterretention ability of the Agrobacterium radiobacter GY04
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Abstract:
A bacterium strain, GY04, was isolated from sand soil of a maize field in suburb of Guiyang. The analysis of DNA sequence suggests that the strain of GY04 is Agrobacterium radiobacter. With the principle of the uniform design, the fermentation conditions of the strain GY04 are optimized. Analyzing by SPSS(V10.0), the optimal fermentation process is determined. They are beef extract 2.000g/L, glycol 2.775 g/L, calcium carbonate 2.000g/L, the initial pH7.000, the inoculum amount 7.000%. On these conditions, the production of the saccharide can be achieved the most, about (2.683±0.600) g/L. The results also show that bacterium A. radiobacter possess good waterretention ability as a microbial waterretaining agent.
