In order to study the rule of drivers’ pupil changing with luminance under road lighting condition,pupil area of 4 subjects under 13 lighting levels of high pressure sodium(HPS), metal halide(MH) and LED are measured, with reaction time test equipment for road lighting and iView X eye tracking system. The results indicate that the relationship between pupil area and background luminance is negative exponential function, pupil will shrink while luminance is increased, the shrink velocity of pupil will decrease while luminance’s increasing, and pupil area will tends to be constant when luminance reach some level. Under the three lighting sources used in the experiment, pupil area will fluctuate while the background luminance is between 0.7 cd/m2and 1.2 cd/m2.