WAN Qiao-ling
Key Laboratory of Three Gorges Reservoir Region’s Eco-Environment, Ministry ofEducation, Chongqing University, Chongqing 400045, P.R.ChinaYANG Chun
Key Laboratory of Three Gorges Reservoir Region’s Eco-Environment, Ministry ofEducation, Chongqing University, Chongqing 400045, P.R.ChinaZHANG Zhi
Key Laboratory of Three Gorges Reservoir Region’s Eco-Environment, Ministry ofEducation, Chongqing University, Chongqing 400045, P.R.ChinaLIAN Peng
Key Laboratory of Three Gorges Reservoir Region’s Eco-Environment, Ministry ofEducation, Chongqing University, Chongqing 400045, P.R.ChinaYI Zhong-hong
Chongqing Pharmaceutical Research Institute Co., Ltd., Chongqing 400061, P.R.ChinaEstrone(E1), 17β-estradiol(E2) and 17α-ethinylestradiol(EE2) exhibit the highest estrogenic potential. A HPLC-MS-MS analysis method using internal standard is established with the multiple reaction monitoring (MRM) model. Further more, the fragment voltages and collision energies in the MRM model are optimized by response surface method (RSM). The optimal fragment voltages of E1, E2 and EE2 are 205.0, 196.0 and 182.0 V respectively and the collision energies are 43.0, 51.0 and 54.0 V. The established method followed the two-step solid phase extraction (SPE) is then applied for the quantification of E1, E2 and EE2 in the water samples collected from a activated sludge process sewage treatment work and Jialing River at the urban section of Chongqing City. The detection limits of E1, E2 and EE2 are 0.5, 0.4 and 0.1 ng/L.
万巧玲,阳春,张智,廉鹏,易中宏.响应曲面法在类固醇雌激素HPLC-MS-MS定量分析中的应用[J].重庆大学学报,2011,34(5):105~111
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