To explore the vibrating frequencies and rational impact factors of multi-span skew bridge, the recursive algorithm of vibrating frequency and finite element formulations of static and dynamic analysis are established for uniform section multi-span continuous skew bridge. The first five order frequency changes of equal-span multi-span continuous skew bridge and unequal-span multi-span skew bridge are compared. The interaction of vehicle-bridge system for two-span skew bridge is analyzed. The history curves for deflection and moment of middle-span are presented. The influence of obliquity on dynamic deflection and dynamic moment as well as the influence of vehicle velocity and obliquity on impact factors of deflection and moment are investigated. Results show that the fundamental frequency of multi-span skew bridge is identical to that of single span skew bridge. The higher order frequencies of single span skew bridge are distributed in frequencies of multi-span skew bridge, which mix other vibrating frequencies. The more the span number is, the more the other vibrating frequencies mingle. Obliquity has an important influence on dynamical characteristics of skew bridge. Impact effects of vehicle have no harmonious changing rule with vehicle velocity and obliquity. The impact factors of deflection and moment are different for skew bridge.