Dielectric barrier discharge (DBD) can produce homogenous discharge in normal temperature and atomsphere with low energy consumption, so it has broad development prospects in industrial applications. An experimental study is carried out to optimize the needles arrangement and supply parameters aiming at the multineedle-to-cylinder reactor with orthogonal design in combination with Lissajous figures. As it is konwn, the process of discharge is also the process of charge transfer; hence, the more charges are transported, the higher reaction efficiency in plasma is. The orthogonal design is established with transported charges as the goal factor, and supply voltage, supply frequency, needles length(NL), interneedle rotation angles(INRA) as factors. Variance analysis results show that the largest influence factor is supply voltage, followed by supply frequency; the electrode configuration of NL=(3.5+2) mm and INRA=45° matched with f=21 kHz makes transported charges per cycle biggest. The orthogonal experiment results are verified by further experiments, which is significant to improve the DBD plasma reaction efficiency.