Car interior noise induced by windshield wiper system is tested for four different types of cars under high and low wiping speeds,and sound samples for subjective annoyance study are prepared. Grouped pair-wise comparison is adopted for the subjective evaluation test. The characteristic of subjective values and relation of four psychoacoustic parameters to the subjective annoyance are analyzed in two wiping speeds and various wiping patterns. The results indicate that the annoyance caused by wiper noise at high speed is higher than that at low speed,and downwards wiping noise is more annoying than upwards wiping noise. The work concluded that the subjective annoyance of noise from wiper system could be described by binaural loudness and fluctuation.