Selective laser melting (SLM), a kind of layer by layer processing technology, has significant performance advantages on preparation of porous materials. SLM provides an effective method to reduce the elastic modulus and the stress shielding effect of TC4 titanium alloys for medical implants.However, there is still no convincing biological safety evaluation before practical application. In this paper, TC4 alloy with a relative density of 99.5% is prepared in the adoption of SLM.We examine corrosion resistance and biocompatibility for TC4 alloy prepared by SLM via electrochemical, hemolysis and cytotoxicity test.Comparison between TC4 alloy prepared by SLM and traditional cast rolling process is discussed.The results show that TC4 alloy prepared by SLM has better corrosion resistance and more excellent biocompatibility than traditional process, and it has tremendous application in futures on biomedical implants.