A transmission line model was established by using 55 human arterial trees to simulate the propagation of pulse wave in human arterial tree, and the effect of resistance change was investigated by changing the peripheral resistance of the model branch under the condition of the settled volume flow rate wave of cardiac output. The results show that with the increase of the peripheral resistance of a branch, its blood flow rate drops rapidly, the rise of the average blood pressure of the aorta and radial artery can be caused by the increase of the resistance ratio of different organs, and the trend of the elevation decreases gradually. When η is set as 4, the radial artery mean pressure is 2.86%,4.55%,1.90%,12.15%,9.81% and 1.49% higher than normal respectively with the flow resistance increase of liver, spleen, stomach, superior mesenteric, right kidney and inferior mesenteric branch. The objective relationship between pulse wave harmonics and the pathological changes of the organs is the basis of pulse diagnosis in traditional Chinese medicine. By analyzing the pulse wave frequency domain of radial artery, it is found that the connection between the amplitude of pulse wave and the change of peripheral resistance of each organ is not obvious. And the effect of cardiac self-regulation with different organ lesions should be considered in the simulation.