To investigate the random parameter sensitivity of reinforced concrete frame structure, Matlab and SAP2000 were used to program Sobol' method and randomized Pushover method. One plane frame structure was established to analyze the sensitivity of parameters to roof displacement. The parameters contained maximum value of horizontal earthquake influence coefficient, concrete compressive strength, elastic modulus of concrete, yield strength and tensile strength of steel bar and elastic modulus of steel bar. The results showed that the most sensitive and important parameter to roof displacement in randomized Pushover method was maximum value of horizontal earthquake influence coefficient and the randomness of remaining five parameters could be ignored in the analysis. In addition, two examples were used to verify the applicability of the findings in the multi-layer and high-rise frame structures.