In order to study the role of endothelial cell subsets in the blood-brain barrier of patients with autism spectrum disorder (ASD), a series of data mining methods were used to analyze the data set of single nuclei RNA sequencing in prefrontal cortex of ASD patients. First of all, the endothelial cells were divided into 4 subsets using graph clustering method. Then, the differentially expressed genes of each subset in ASD state were obtained by Wilcoxon rank sum test, KEGG enrichment and GO analysis were carried out. The results indicated that endothelial cell subset 1 was mainly involved in the changes of ATP synthesis and metabolism, mitochondria and activity of various enzymes such as oxidoreductase. Finally, the cell crosstalk between endothelial cell subsets and other cell groups in brain tissue was analyzed. The results showed that the number of endothelial cells receiving microglial signals increased in ASD patients; There may be specific SPP1 - (ITGAV+ITGB1) and SPP1 - (ITGAV+ITGB5) ligand receptor pair signals between endothelial cell subset 1 and microglia; At the same time, the number and strength of astrocyte signals received decreased.